Jee Eun Han - Page 2 of 2 - Responsible Seafood Advocate

PCR methods characterize AHPND V.p isolates

In analyzing the plasmid sequence from the whole genome sequences of AHPND V. parahaemolyticus (V.p) isolates, researchers identified a clear geographical variation within the plasmid, and developed PCR methods to characterize AHPND V.p isolates as either Mexico-type or Southeast Asia-type.

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Health & Welfare

Real-time PCR offers sensitivity, specificity in detecting AHPND plasmid

Real-time, quantitative polymerase chain reaction (qPCR) analysis has become an attractive alternative for quantification of the acute hepatopancreatic necrosis disease (AHPND) pathogen in infected shrimp. The authors developed a qPCR assay to quantify a virulence plasmid harbored by the bacterium that causes AHPND.